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crebbp bromodomain  (Addgene inc)


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    Addgene inc crebbp bromodomain
    Crebbp Bromodomain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crebbp+bromodomain/CREBBP+(Plasmid+%2338977)/pm34255515__jm1c00348_si_001-79-1-3
    Average 93 stars, based on 12 article reviews
    crebbp bromodomain - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously4,5. .. The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) construct was transformed into E. coli BL21 (DE3) cells for expression, as described previously. .. [11] The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Article Title: Selective Fragments for the CREBBP Bromodomain Identified from an Encoded Self-assembly Chemical Library.
    Article Snippet: DNA-encoded chemical libraries (DECLs) are collections of chemical moieties individually coupled to distinctive DNA barcodes.. Compounds can be displayed either at the end of a single DNA strand (i. e., single-pharmacophore libraries) or at the extremities of two complementary DNA strands (i. e., dualpharmacophore libraries).. In this work, we describe the use of a dual-pharmacophore encoded self-assembly chemical (ESAC) library for the affinity maturation of a known 4,5-dihydrobenzodiazepinone ring (THBD) acetyl-lysine (KAc) mimic for the cyclicAMP response element binding protein (CREB) binding protein (CREBBP or CBP) bromodomain.

    Construct:

    Article Title: Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously4,5. .. The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) construct was transformed into E. coli BL21 (DE3) cells for expression, as described previously. .. [11] The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Article Title: Selective Fragments for the CREBBP Bromodomain Identified from an Encoded Self-assembly Chemical Library.
    Article Snippet: DNA-encoded chemical libraries (DECLs) are collections of chemical moieties individually coupled to distinctive DNA barcodes.. Compounds can be displayed either at the end of a single DNA strand (i. e., single-pharmacophore libraries) or at the extremities of two complementary DNA strands (i. e., dualpharmacophore libraries).. In this work, we describe the use of a dual-pharmacophore encoded self-assembly chemical (ESAC) library for the affinity maturation of a known 4,5-dihydrobenzodiazepinone ring (THBD) acetyl-lysine (KAc) mimic for the cyclicAMP response element binding protein (CREB) binding protein (CREBBP or CBP) bromodomain.

    Transformation Assay:

    Article Title: Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously4,5. .. The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) construct was transformed into E. coli BL21 (DE3) cells for expression, as described previously. .. [11] The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Article Title: Selective Fragments for the CREBBP Bromodomain Identified from an Encoded Self-assembly Chemical Library.
    Article Snippet: DNA-encoded chemical libraries (DECLs) are collections of chemical moieties individually coupled to distinctive DNA barcodes.. Compounds can be displayed either at the end of a single DNA strand (i. e., single-pharmacophore libraries) or at the extremities of two complementary DNA strands (i. e., dualpharmacophore libraries).. In this work, we describe the use of a dual-pharmacophore encoded self-assembly chemical (ESAC) library for the affinity maturation of a known 4,5-dihydrobenzodiazepinone ring (THBD) acetyl-lysine (KAc) mimic for the cyclicAMP response element binding protein (CREB) binding protein (CREBBP or CBP) bromodomain.

    Expressing:

    Article Title: Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously4,5. .. The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. The CREBBP bromodomain (Addgene plasmid # 38977) construct was transformed into E. coli BL21 (DE3) cells for expression, as described previously. .. [11] The proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare).

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Article Title: Selective Fragments for the CREBBP Bromodomain Identified from an Encoded Self-assembly Chemical Library.
    Article Snippet: DNA-encoded chemical libraries (DECLs) are collections of chemical moieties individually coupled to distinctive DNA barcodes.. Compounds can be displayed either at the end of a single DNA strand (i. e., single-pharmacophore libraries) or at the extremities of two complementary DNA strands (i. e., dualpharmacophore libraries).. In this work, we describe the use of a dual-pharmacophore encoded self-assembly chemical (ESAC) library for the affinity maturation of a known 4,5-dihydrobenzodiazepinone ring (THBD) acetyl-lysine (KAc) mimic for the cyclicAMP response element binding protein (CREB) binding protein (CREBBP or CBP) bromodomain.

    Purification:

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Affinity Chromatography:

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Filtration:

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..

    Chromatography:

    Article Title: Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain
    Article Snippet: Plasmid: CREBBP (Addgene #38977) Vector backbone: pNIC28-Bsa4 (Addgene #26103) Protein Expression and Purification. .. The CREBBP bromodomain (Addgene plasmid # 38977) and BRD4(1) bromodomain (Addgene plasmid #38942) constructs were transformed into E. coli BL21 (DE3) cells for expression, as described previously.1,14 The S22 proteins were purified using Immobilized Metal Affinity Chromatography (IMAC) with an HisTrapTM column (GE Healthcare) followed by gel filtration chromatography with Superdex 75 resin (GE Healthcare). ..



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    Image Search Results


    Figure 1. Acetylated histone-binding site, “WPF” shelf tryptophan (red), and a basic interaction site (blue) for (A) BRDT−BD1 (W50, K37, K41, and K45) and (B) BRD4−BD1 (W81, R68, K72, and K76). (C) Sequence similarity in each bromodomain of BRDT (left) and BRD4 (right) relative to BRD4−BD1. Percent similarities were relative to BRD4−BD1 calculated by the PDB sequence and structure alignment (PDB ID: 7L73 for BRDT−BD1 and 3UVW for BRD4−BD1).

    Journal: Biochemistry

    Article Title: Alternative Mechanisms for DNA Engagement by BET Bromodomain-Containing Proteins.

    doi: 10.1021/acs.biochem.2c00157

    Figure Lengend Snippet: Figure 1. Acetylated histone-binding site, “WPF” shelf tryptophan (red), and a basic interaction site (blue) for (A) BRDT−BD1 (W50, K37, K41, and K45) and (B) BRD4−BD1 (W81, R68, K72, and K76). (C) Sequence similarity in each bromodomain of BRDT (left) and BRD4 (right) relative to BRD4−BD1. Percent similarities were relative to BRD4−BD1 calculated by the PDB sequence and structure alignment (PDB ID: 7L73 for BRDT−BD1 and 3UVW for BRD4−BD1).

    Article Snippet: The pNIC28-Bsa4 plasmid containing the first bromodomain of BRD4 (Addgene #38943), first bromodomain of BRDT (Addgene #38898), second bromodomain of BRD2 (Addgene https://doi.org/10.1021/acs.biochem.2c00157 Biochemistry 2022, 61, 1260−1272 1261 #39074), bromodomain of CREB-binding protein (Addgene #38977), and bromodomain of BPTF (Addgene #39111) were a kind gift from Nicola Burgess-Brown.

    Techniques: Binding Assay, Sequencing

    Figure 6. (A) Bromodomain phylogenetic tree adapted from Pomerantz et al..37 BDs tested in the PrOF NMR screen are highlighted. (B) Table showing PrOF NMR Kd values from the titration of 40 bp dsDNA with 5FW-labeled proteins. N.B. = binding affinity could not be determined. a: Kd is reported as the mean ± SD of two experimental replicates. b: Kd is reported as the mean ± SD of three experimental replicates.

    Journal: Biochemistry

    Article Title: Alternative Mechanisms for DNA Engagement by BET Bromodomain-Containing Proteins.

    doi: 10.1021/acs.biochem.2c00157

    Figure Lengend Snippet: Figure 6. (A) Bromodomain phylogenetic tree adapted from Pomerantz et al..37 BDs tested in the PrOF NMR screen are highlighted. (B) Table showing PrOF NMR Kd values from the titration of 40 bp dsDNA with 5FW-labeled proteins. N.B. = binding affinity could not be determined. a: Kd is reported as the mean ± SD of two experimental replicates. b: Kd is reported as the mean ± SD of three experimental replicates.

    Article Snippet: The pNIC28-Bsa4 plasmid containing the first bromodomain of BRD4 (Addgene #38943), first bromodomain of BRDT (Addgene #38898), second bromodomain of BRD2 (Addgene https://doi.org/10.1021/acs.biochem.2c00157 Biochemistry 2022, 61, 1260−1272 1261 #39074), bromodomain of CREB-binding protein (Addgene #38977), and bromodomain of BPTF (Addgene #39111) were a kind gift from Nicola Burgess-Brown.

    Techniques: Titration, Labeling, Binding Assay

    (A) Representative flow-cytometric analysis of splenic B220+ cells from Crebbp+/+, Crebbpfl/+, and Crebbpfl/fl (top) vs Ep300+/+, Ep300fl/+, and Ep300fl/fl (bottom) Cγ1Cre/+ mice, analyzed 10 days after SRBC immunization. GC B cells are identified as CD95+PNAhi cells, and numbers in each image indicate the percentage in the gate.

    Journal: Immunity

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma

    doi: 10.1016/j.immuni.2019.08.006

    Figure Lengend Snippet: (A) Representative flow-cytometric analysis of splenic B220+ cells from Crebbp+/+, Crebbpfl/+, and Crebbpfl/fl (top) vs Ep300+/+, Ep300fl/+, and Ep300fl/fl (bottom) Cγ1Cre/+ mice, analyzed 10 days after SRBC immunization. GC B cells are identified as CD95+PNAhi cells, and numbers in each image indicate the percentage in the gate.

    Article Snippet: CCS1477, a selective and orally active small molecule inhibitor of the single bromodomain of CREBBP and EP300, was obtained from CellCentric ( Pegg et al., 2017 ).

    Techniques:

    (A) Representative flow-cytometric analysis of splenic B220+ cells from Crebbp+/+Ep300+/+, Crebbpfl/+Ep300fl/+, Crebbpfl/+Ep300fl/fl, Crebbpfl/flEp300fl/+, and Crebbpfl/flEp300fl/fl Cγ1Cre/+ mice, analyzed 10 days after SRBC immunization. GC B cells are identified as CD95+PNAhi cells, and numbers in each image indicate the percentage in the gate.

    Journal: Immunity

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma

    doi: 10.1016/j.immuni.2019.08.006

    Figure Lengend Snippet: (A) Representative flow-cytometric analysis of splenic B220+ cells from Crebbp+/+Ep300+/+, Crebbpfl/+Ep300fl/+, Crebbpfl/+Ep300fl/fl, Crebbpfl/flEp300fl/+, and Crebbpfl/flEp300fl/fl Cγ1Cre/+ mice, analyzed 10 days after SRBC immunization. GC B cells are identified as CD95+PNAhi cells, and numbers in each image indicate the percentage in the gate.

    Article Snippet: CCS1477, a selective and orally active small molecule inhibitor of the single bromodomain of CREBBP and EP300, was obtained from CellCentric ( Pegg et al., 2017 ).

    Techniques:

    (A) WB analysis of Crebbp and Ep300 expression in Ficoll-separated splenic B cells of the indicated genotypes, cultured ex vivo in the presence of αCD40 and IL-4 for 4 days. Analysis of H3K27Ac and H3K18Ac monitors for the functional effects of Crebbp and/or Ep300 loss and is quantified on the bottom. Tubulin and total H3 serve as loading control for whole-cell and chromatin extracts, respectively.

    Journal: Immunity

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma

    doi: 10.1016/j.immuni.2019.08.006

    Figure Lengend Snippet: (A) WB analysis of Crebbp and Ep300 expression in Ficoll-separated splenic B cells of the indicated genotypes, cultured ex vivo in the presence of αCD40 and IL-4 for 4 days. Analysis of H3K27Ac and H3K18Ac monitors for the functional effects of Crebbp and/or Ep300 loss and is quantified on the bottom. Tubulin and total H3 serve as loading control for whole-cell and chromatin extracts, respectively.

    Article Snippet: CCS1477, a selective and orally active small molecule inhibitor of the single bromodomain of CREBBP and EP300, was obtained from CellCentric ( Pegg et al., 2017 ).

    Techniques: Expressing, Cell Culture, Ex Vivo, Functional Assay, Control

    (A) Immunoblot analysis of EP300 and Cas9 expression in five DLBCL cell lines carrying wild-type (wt/wt) or mutant (M indicates truncating mutation; m indicates missense mutation or in frame deletion) CREBBP alleles, treated with Dox for 3 days to induce Cas9-mediated disruption of the EP300 gene or a control intronic region. Values indicate normalized EP300 protein levels in relation to uninduced, set as 1; α-Tubulin, loading control.

    Journal: Immunity

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma

    doi: 10.1016/j.immuni.2019.08.006

    Figure Lengend Snippet: (A) Immunoblot analysis of EP300 and Cas9 expression in five DLBCL cell lines carrying wild-type (wt/wt) or mutant (M indicates truncating mutation; m indicates missense mutation or in frame deletion) CREBBP alleles, treated with Dox for 3 days to induce Cas9-mediated disruption of the EP300 gene or a control intronic region. Values indicate normalized EP300 protein levels in relation to uninduced, set as 1; α-Tubulin, loading control.

    Article Snippet: CCS1477, a selective and orally active small molecule inhibitor of the single bromodomain of CREBBP and EP300, was obtained from CellCentric ( Pegg et al., 2017 ).

    Techniques: Western Blot, Expressing, Mutagenesis, Disruption, Control

    KEY RESOURCES TABLE

    Journal: Immunity

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma

    doi: 10.1016/j.immuni.2019.08.006

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CCS1477, a selective and orally active small molecule inhibitor of the single bromodomain of CREBBP and EP300, was obtained from CellCentric ( Pegg et al., 2017 ).

    Techniques: Plasmid Preparation, CRISPR, Amplification, Recombinant, Cell Isolation, Cell Viability Assay, Western Blot, RNA Library Preparation, Magnetic Beads, Software